FIGURE 5.

Inhibition of initiation of translation alleviates folding defects of mutant CFTR. A, Ussing chamber assay: hippuristanol concentration effect on CFTR-mediated short-circuit currents recorded in duplicates from FRT cell monolayers incubated for 24 h with the inhibitor. The experiment was done as described in the legend to Fig. 4A. B, effect of hippuristanol on expression of F508del-CFTR. The levels of F508del-CFTR in cells were measured as described in the legend to Fig. 4C. Band C, mature form; Band B, core-glycosylated form. Error bars represent S.E.