TABLE 1.

SAXS parameters for uPAR

R_g^autoRg and R_g^GNOM are the radii of gyration estimated from the SAXS data using the automated Guinier analysis routine and GNOM, respectively. D_max, V_p, and MM_SAXS are the maximum particle dimensions, hydrated particle volumes, and molecular masses estimated from I(0), respectively. Extracted parameters are taken from the merged SAXS curve from all data sets and extrapolated to infinite dilution, and the molecular masses calculated from the sequence (including four standard glycosylations) are shown (MM_seq). The experimentally determined masses for the glycosylated uPAR^wt and uPAR^H47C/N259C preparations used in this study are both within 35.1–35.4 kDa, where the differences from the calculated masses most likely relate to incomplete fucosylations of the innermost chitobiose moieties of the glycans.

Protein	RgautoRg	RgGNOM	Dmax	Vp	MMSAXS	MMseq
	nm	nm	nm	nm3	kDa	kDa
uPARwt	2.8 ± 0.1	2.9 ± 0.1	9.7 ± 0.5	69 ± 10	47 ± 5	35.6
uPARH47C/N259C	2.2 ± 0.1	2.2 ± 0.1	7.0 ± 0.5	57 ± 10	34 ± 5	35.6
uPARwt·AE105	2.5 ± 0.1	2.5 ± 0.1	8.5 ± 0.5	61 ± 5	37 ± 5	36.8
uPARwt·AE234	2.4 ± 0.1	2.4 ± 0.1	8.3 ± 0.5	57 ± 5	42 ± 5	36.9
uPARwt·ATF	2.8 ± 0.1	2.8 ± 0.1	9.4 ± 0.5	102 ± 10	58 ± 5	51.8