FIGURE 1.
Structural analysis of Mma_DMAG. A, a TLC profile of purified arabino-glycero lipids from Mma shows the major compound identified as di-mycolyl di-arabino-glycerol (Mma_DMAG) and two minor related glycoconjugates identified as mono-mycolyl mono-arabino-glycerol (X1) and mono-mycolyl di-arabino-glycerol (X2). Rf are indicated in the left margin. MALDI-TOF-MS spectrum of intact Mma_DMAG (B) and mycolic acid methyl esters (MAMEs) (C) derived from Mma_DMAG show the presence of α-, keto-, and methoxy-mycolic acids. D, 1H NMR spectrum of Mma_DMAG; a to n indicate the relevant signals used for the identification of functional groups of mycolates.