TABLE 2.
Microarray analyses; genes up-regulated in human macrophages-like THP1 cells after stimulation for 8 h with Mma_DMAG
The complete list of altered gene expression was available in supplemental Table S1.
| Gene | Description | Log2 ratioa |
|---|---|---|
| Cell surface antigen | ||
| ICAM1 | Intercellular adhesion molecule 1 | 4.20 |
| VCAM1 | Vascular cell adhesion molecule 1 | 2.84 |
| CD40 | CD40 molecule, TNF receptor superfamily member 5 | 2.49 |
| CD83 | CD83 molecule | 3.06 |
| CD44 | CD44 molecule (Indian blood group) | 2.07 |
| ITGB8 | Integrin, β8 | 2.04 |
| SLAMF7 | Signaling lymphocytic activation molecule family member 7 | 3.86 |
| Protease/metalloproteinase | ||
| MMP1 | Matrix metallopeptidase 1 (interstitial collagenase) | 4.95 |
| MMP9 | Matrix metallopeptidase9 (gelatinase B, 92-kDa gelatinase, 92-kDa type IV collagenase) | 3.25 |
| PCSK5 | Proprotein convertase subtilisin/kexin type 5 | 2.91 |
| Chemokines-cytokines | ||
| CCL8 (MCP-2) | Chemokine ligand 8 | 5.94 |
| CCL2 (MCP-1) | Chemokine ligand 2 | 4.91 |
| CXCL2 (MIP-2) | Chemokine ligand 2 | 4.62 |
| CXCL1 | Chemokine ligand 1 | 4.31 |
| CCL4 (MIP-1) | Chemokine ligand 4 | 4.07 |
| CCL20 | Chemokine ligand 20 | 4.00 |
| CXCL11 | Chemokine ligand 11 | 3.70 |
| CXCL3(GRO) | Chemokine ligand 3 | 3.65 |
| CXCL10 | Chemokine ligand 10 | 3.58 |
| CCL7 | Chemokine ligand 7 | 3.41 |
| CCL3L3 | Chemokine ligand 3-like 3 | 3.37 |
| CXCL9 | Chemokine ligand 9 | 2.02 |
| IL-8 | Interleukin 8 | 4.11 |
| IL-1β | Interleukin 1, β | 5.54 |
| TNFα | Tumor necrosis factor | 3.33 |
| IL-1α | Interleukin 1, α | 2.42 |
| Cytokine receptors | ||
| CCR7 | Chemokine (C-C motif) receptor 7 | 4.18 |
| IL18RAP | Interleukin 18 receptor accessory protein | 3.17 |
| CMKLR1 | Chemokine-like receptor 1 | 2.37 |
| Apoptosis | ||
| BCL2A1 | BCL2-related protein A1 | 3.34 |
| TNFAIP3 | Tumor necrosis factor, α-induced protein 3 | 3.25 |
| IER3 | Immediate early response 3 | 3.33 |
| Redox-enzymes | ||
| CYP19A1 | Cytochrome P450, family 19, subfamily A, polypeptide 1 | 3.27 |
| SOD2 | Superoxide dismutase 2, mitochondrial | 3.18 |
| Cell signaling pathways/nuclear factors | ||
| RGS1 | Regulator of G-protein signaling 1 | 4.01 |
| NR4A3 | Nuclear receptor subfamily 4, group A, member 3 | 3.92 |
| RND3 | Rho family GTPase 3 | 3.70 |
| TNIP3 | TNFAIP3 interacting protein 3 | 3.23 |
| TNFAIP3 | Tumor necrosis factor, α-induced protein 3 | 3.25 |
| RCAN1 | Regulator of calcineurin 1 | 3.16 |
| SOCS3 | Suppressor of cytokine signaling 3 | 3.09 |
| DUSP1 | Dual specificity phosphatase 1 | 3.04 |
| RGS16 | Regulator of G-protein signaling 16 | 2.96 |
| STAT4 | Signal transducer and activator of transcription 4 | 2.73 |
| ATF3 | Activating transcription factor 3 | 2.75 |
| TRAF1 | TNF receptor-associated factor 1 | 2.68 |
| BCL3 | B-cell CLL/lymphoma 3 | 2.28 |
| SPHK1 | Sphingosine kinase 1 | 2.16 |
| NFKB2 | Nuclear factor of κ light polypeptide gene enhancer | 2.10 |
| NFKB1 | Nuclear factor of κ light polypeptide gene enhancer | 2.19 |
| NFKBIZ | Nuclear factor of κ light polypeptide gene enhancer | 2.46 |
| IFN-inducible protein | ||
| IFIT3 | Interferon-induced protein | 3.24 |
| IFIT2 | Interferon-induced protein | 2.50 |
| IFIT1 | Interferon-induced protein | 2.4 |
| IFIT5 | Interferon-induced protein | 2.17 |
| Transporters/channels | ||
| AQP9 | Aquaporin 9 | 3.66 |
| SLC7A11 | Solute carrier family 7 | 2.51 |
| SLC7A11 | ATPase, Ca2+ transporting, plasma membrane 1 | 2.27 |
| Lipidb and amino acidc metabolism | ||
| FABP4b | Fatty acid-binding protein 4 | 3.38 |
| AGPAT9b | 1-Acylglycerol-3-phosphate O-acyltransferase 9 | 3.13 |
| PTGS2b | Prostaglandin-endoperoxide synthase 2 | 3.17 |
| MGLLb | Monoglyceride lipase | 3.07 |
| PLA2G7b | Phospholipase A2, group VII | 2.78 |
| ACSL1b | Acyl-CoA synthetase long-chain family member 1 | 2.36 |
| LRP12b | Low density lipoprotein receptor-related protein 12 | 2.11 |
| IDO1c | Indoleamine 2,3-dioxygenase 1 (Trp catabolism) | 2.78 |
| KYNUc | Kynureninase (l-kynurenine hydrolase) | 2.36 |
| Carbohydrate metabolism | ||
| CHST7 | Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 | 2.42 |
| CHST2 | Carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 | 2.12 |
| NEU4 | Sialidase 4 | 2.26 |
| SDC4 | Syndecan 4 | 2.22 |
| Nucleic metabolism | ||
| PDE4B | Phosphodiesterase 4B, cAMP-specific | 2.59 |
| NTSE | 5′-Nucleotidase, ecto (CD73) | 2.35 |
| NAMPT | Nicotinamide phosphoribosyltransferase | 2.27 |
| ADORA2A | Adenosine A2a receptor | 2.85 |
| ADAD2 | Adenosine deaminase domain containing 2 | 2.83 |
| Miscellaneous | ||
| EBI3 | Epstein-Barr virus-induced 3 | 3.65 |
| EGR2 | Early growth response 2 | 3.29 |
| SERPINE2 | Serpin peptidase inhibitor, serpin peptidase inhibitor, cladeE (nexin, plasminogen activator inhibitor type 1), member 2 | 3.24 |
| HBEGF | Heparin binding EGF-like growth factor | 2.03 |
a Binary log ratio of altered gene expression according to microarray analysis of macrophages after exposure for 8 h with DMAG compared to unstimulated cells. Results with a log2 ratio >2 are shown and are representative of two independent experiments.
b Genes related to lipid metabolism.
c Genes related to amino acid metabolism.