FIGURE 2.

Role of NLRs and adaptor molecules in caspase-1 activation and IL-1β secretion from macrophages infected with Gram-negative bacteria. Bone marrow-derived macrophages from WT, Asc^−/−, Aim2^−/−, Nlrp3^−/−, Nlrc4^−/−, Nod1^−/−, Nod2^−/−, Nlrp6^−/−, and Nlrp12^−/− mice were infected with C. rodentium (m.o.i. 20), E. coli (m.o.i. 20), or S. typhimurium (m.o.i. 5) for 24 h as described under “Experimental Procedures.” Caspase-1 activation was determined by Western blotting of cell lysates (A, C, and E), and secreted IL-1β in supernatants of infected macrophages was quantified by ELISA (B, D, and F). Data represent means ± S.E. (error bars) and are representative of at least three independent experiments.