FIGURE 8.

Effect of the mini-TMD antibodies on the HinR activating and inactivating mutations of TSHR and LHR. A and B, flow cytometric analysis of HEK293 cells transfected with the WT or different HinR mutants of LHR (panel A) or TSHR (panel B) using the HinR-specific antibodies (10 μg/ml) and the mini-TMD antibodies (1 μg/ml) is shown. Accessibility of epitopes to the mini-TMD antibodies to the HinR mutants was estimated by Ra of the mutants with respect to the WT LHR (Aii) or TSHR (Bii) as mentioned in the legend of Fig. 7A. C and D, the hormone-stimulated and the basal cAMP production of LHR CAM S277Q (C) and TSHR HinR mutants S281I and D410N (D) was measured in the presence of NRIgG or the mini-TMD antibodies (10 μg/ml). hTSH, human TSH.