FIGURE 9.

Effect of the mini-TMD antibodies on the activating mutations of TSHR ECLs and LHR TMD. Flow cytometric analysis of HEK293 cells transfected with the WT or TSHR ECL CAMs (Ai) using the mini-TMD antibodies (1 μg/ml) and the RMFI values so obtained were normalized to those using the HinR-specific antibodies. Basal cAMP production of TSHR ECL CAM (Aii), LHR TMD CAM D578Y (Bi), and L457R (Bii) was estimated in the presence of 10 μg/ml of preimmune IgG (gray bars) or mini-TMD IgG (black bars).