TABLE 2.
Inhibitory activity of CP621-652 and its mutants on HIV-1 infection
The assays were performed in triplicate and repeated at least three times. The data are expressed as mean ± S.D.
| Peptide | Cell fusion |
Virus entry |
6-HB formation |
|||
|---|---|---|---|---|---|---|
| IC50 | n-Folda | IC50 | n-Fold | IC50 (μm) | n-Fold | |
| nm | nm | μm | ||||
| CP621-652 | 12.0 ± 3.6 | 1 | 6. 9 ± 1.9 | 1 | 7.5 ± 2.0 | 1 |
| Q621A | 14.3 ± 0.3 | 1.2 | 6.3 ± 0.4 | 0.9 | 4.6 ± 1.6 | 0.6 |
| I622A | 14.3 ± 0.9 | 1.2 | 8.0 ± 0.8 | 1.2 | 4.7 ± 0.4 | 0.6 |
| W623A | 11.1 ± 0.9 | 0.9 | 9.1 ± 0.5 | 1.3 | 6.1 ± 0.2 | 0.8 |
| N624A | 13.4 ± 0.9 | 1.1 | 8.0 ± 0.3 | 1.2 | 3.0 ± 0.2 | 0.4 |
| N625A | 15.9 ± 1.0 | 1.3 | 11.7 ± 0.7 | 1.7 | 11.0 ± 2.6 | 1.5 |
| M626A | 52.0 ± 10.3 | 4.3 | 59.3 ± 1.1 | 8.6 | >200 | >26.7 |
| T627A | 67.7 ± 8.7 | 5.6 | 163.9 ± 11.4 | 23.8 | >200 | >26.7 |
a Data were compared with the IC50 values of CP621-652.