FIGURE 11.

EMT inhibitors block [³H]AEA release from U937 cells preloaded with 100 nm [³H]AEA plus AEA. Shown are concentration-dependent intracellular [³H]AEA (AEAintr) accumulation and extracellular [³H]AEA released (AEArel) reduction after a 25-min incubation of UCM707 (A), OMDM-2 (B), or LY2183240 (C) in FAAH-blocked U937 cells (obtained by cellular pretreatment with 100 nm URB597 and 1 μm PMSF) preloaded with 100 nm [³H]AEA plus AEA. The values are expressed as a percentage of cellular or extracellular [³H]AEA levels as compared with vehicle-treated control. Data show mean values ± S.E. (error bars) of three independent experiments, each one carried out at least in triplicate. *, p < 0.05; **, p < 0.01, treated versus vehicle-treated control.