FIGURE 12.

EMT inhibitors inhibit [³H]2-AG release from U937 cells preloaded with 1 μm [³H]2-AG plus 2-AG. Shown are concentration-dependent intracellular [³H]2-AG (2-AGintr) accumulation and extracellular [³H]2-AG released (2-AGrel) reduction after a 25-min incubation with UCM707 (A), OMDM-2 (B), or LY2183240 (C) in MAGL-, ABHD-6-, and ABHD-12-blocked U937 cells (obtained by cellular pretreatment with 1 μm JZL184, 10 μm WWL70, and 20 μm tetrahydrolipstatin) preloaded with 1 μm [³H]2-AG plus 2-AG. The values are expressed as a percentage of cellular or extracellular [³H]2-AG levels as compared with vehicle-treated control. Data show mean values ± S.E. (error bars) of three independent experiments, each one carried out at least in triplicate. *, p < 0.05; **, p < 0.01, treated versus vehicle-treated control.