TABLE 3.
IC50 values for AEA uptake inhibition calculated after a 5-min incubation with 100 nm [3H]AEA plus AEA and 20-min pretreatment with URB597 and PMSF alone or in the presence of UCM707 or OMDM-2 in U937 cells
The IC50 values are expressed as a percentage of vehicle-treated control. The values are expressed as mean ± S.E. of three independent experiments, each one carried out at least in triplicate.
| Compounds/combinations | AEA uptake inhibition (IC50) ± S.E. |
|---|---|
| nm | |
| URB597 | 2.3 ± 0.03 |
| URB597 + UCM707 (1 μm) | <0.01 ± 0.002 |
| URB597 + OMDM-2 (5 μm) | 0.01 ± 0.002 |
| PMSF | 123.1 ± 6.3 |
| PMSF + UCM707 (1 μm) | 1.0 ± 0.07 |
| PMSF + OMDM-2 (5 μm) | <1.0 ± 0.01 |