FIGURE 8.

PKCϵ levels alter GTPP-induced preconditioning against OGD/R-induced cell death. A, overexpression of PKCϵ increases GTPP-induced preconditioning. PC12 cells stably transfected with a metallothionein-driven PKCϵ expression vector were used to overexpress PKCϵ. Cells transfected with an empty vector were used as controls. B, knock-out of PKCϵ decreases GTPP-induced preconditioning. Every 24 h siRNA oligonucleotides were added to the culture medium. The values are shown as the mean ± S.E. for three experiments. Statistical difference between means is indicated with an asterisk (*, p < 0.01).