FIGURE 5.

Cross-inhibition of IgE binding among Blo t 21, Blo t 5, and Der p 5. End point cross-inhibition experiments of IgE binding were conducted using sera from six patients preadsorbed with 100 μg/ml of inhibitor protein. The bars represent percentages of inhibition of IgE binding. Black, gray, and white bars indicate the serum preadsorbed with GST-tagged Blo t 21, Blo t 5, and Der p 5, respectively. Specific IgE binding experiments were performed with plates coated with Blo t 21, Blo t 5, or Der p 5 using the sera preadsorbed with different inhibitor proteins. The averages of two independent experiments with duplicates were plotted with their standard deviations.