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. 2012 Aug 13;287(41):34809–34824. doi: 10.1074/jbc.M112.372797

FIGURE 9.

FIGURE 9.

Activation of unique PR-B NH2-terminal region by MAGE-11 and p300. A and B, GAL-PR-B-(1–164) (50 ng) was expressed in HeLa cells with 0.1 μg of 5XGAL4Luc3 and 10 or 150 ng of pSG5 empty vector, 10–150 ng of pSG5-MAGE (A), and 10–150 ng of pSG5-HA-p300 (B), and luciferase activity was measured. C, GAL-PR-B-(1–164) (50 ng) was expressed in HeLa cells using Lipofectamine 2000 with 0.1 μg of 5XGAL4Luc3, 0.1 μg of pSG5 empty vector (−), or 0.1 μg of pSG5-HA-p300 with and without 5 nm nonspecific siRNA-3 (NS) and MAGE-11 siRNA-2 or -3. D, GALO empty vector (−) (50 ng) or 50 ng of GAL-PR-B-(1–164) or GAL-PR-B-(456–546) or 10 ng of GAL-PR-B-(1–559) were expressed in HeLa cells with 0.1 μg of 5XGAL4Luc3 and 25 ng of pSG5 empty vector (−) or pSG5-MAGE (M) with and without pSG5-HA-p300 (P). Error bars, S.E.