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. 2001 Jun 12;98(13):7200–7205. doi: 10.1073/pnas.121173298

Figure 4.

Figure 4

Effect of PI(3)K/Akt pathway on the interaction between AR and ARA70. (A) Modulation of interaction between AR and ARA70 by cAkt, dAkt, or LY294002. The DU145 cells were transfected with 2.5 μg of GAL4-ARA70 and 2.5 μg of VP16-AR, followed by treatment with LY294002 or vehicle 30 min before DHT treatment. The interaction between AR and ARA70 was determined by CAT assay by using pG5-CAT as a reporter. (B) The enhanced AR transactivation by various AR coactivators, ARA70, ARA54, TIF2, and SRC-1, could be further promoted in the presence of LY294002 or Δp85. The data are means ± SD from three independent experiments.