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. 2001 Jun 12;98(13):7200–7205. doi: 10.1073/pnas.121173298

Figure 5.

Figure 5

PI(3)K/Akt pathway suppressed androgen/AR-induced apoptosis. (A) PC-3(AR)2 and PC-3(AR)6 expressed AR protein. PC-3 cells were stably transfected with AR, followed by selection with hygromycin B, and confirmed by Western blotting by using AR antibody NH27, whereas LNCaP was used as a positive control. (B) Androgen/AR-induced apoptosis in PC-3(AR)2, PC-3(AR)6, and SAR-91 were inhibited by PI(3)K/Akt pathway. SAR-91, S7MC, PC-3(AR)2, and PC-3(AR)6 were treated with LY294002 (20 μM) or HF (5 μM) for 30 min, followed by addition of IGF-1 (100 ng/ml) for another 30 min before DHT treatment. After 3 days, cell apoptosis was analyzed by TUNEL assay. The data are means ± SD from three independent experiments.