Table 1.
Breed and genotype of individuals sequenced for the presence ofFGF20c.535A > T
| Breed | Genotype | No. of individuals | c.496 (rs14481412) | c.535 | c.552 (rs14481413) |
|---|---|---|---|---|---|
| UC Davis (low line) |
sc/sc |
10 |
CC |
TT |
AA |
| UC Davis (high line) |
sc/sc |
10 |
CC |
TT |
AA |
| Storrs, Connecticut (low line) |
sc/sc |
2 |
CC |
TT |
AA |
| Israeli experimental line |
sc/sc |
16 |
CC |
TT |
AA |
| Israeli experimental line |
sc/+ |
3 |
CC |
AT |
AA |
| Israeli experimental line |
sc/+ |
6 |
CC |
AT |
AG |
| New Hampshire (IFAG, Germany) |
WT |
6 |
CC |
AA |
AA |
| New Hampshire (IFAG, Germany) |
WT |
4 |
CT |
AA |
AG |
| New Hampshire, UK |
WT |
2 |
TT |
AA |
GG |
| New Hampshire, UK |
WT |
1 |
CT |
AA |
AG |
| Black Leghorn |
WT |
1 |
CC |
AA |
AA |
| Araucana |
WT |
1 |
CC |
AA |
AA |
| Old English Pheasant Fowl |
WT |
1 |
CC |
AA |
AA |
| Langshan |
WT |
1 |
CT |
AA |
AG |
| Silver Appenzeller |
WT |
1 |
CC |
AA |
AA |
| Roslin Institute experimental |
WT |
2 |
CC |
AA |
AA |
| Roslin Institute experimental |
WT |
1 |
CT |
AA |
AG |
| ISA Brown |
WT |
2 |
CC |
AA |
AA |
| Outbred, Mexico |
WT |
7 |
CC |
AA |
AA |
| Outbred, Mexico |
WT |
2 |
TT |
AA |
GG |
| Outbred, Mexico |
WT |
1 |
CT |
AA |
AG |
| Cream Legbar |
WT |
1 |
CC |
AA |
AA |
| Dorking |
WT |
1 |
CC |
AA |
AA |
| Campine |
WT |
1 |
CC |
AA |
AA |
| Naked Neck, Wernlas collection |
WT |
1 |
TT |
AA |
GG |
| Hungarian AVIANDIV Naked Neck |
WT |
1 |
TT |
AA |
GG |
| Naked Neck, English |
WT |
1 |
CC |
AA |
AA |
| Scots Grey | WT | 1 | TT | AA | GG |
Results from sequencing FGF20 exon 3 in 38 sc/sc individuals, 9 sc/+ individuals and 40 WT birds. The FGF20 c.535A > T mutation was found in all of the sc/sc and none of the WT samples sequenced. The genotype of the two flanking SNPs (rs14481412 and rs14481413) revealed that 25/40 WT individuals were homozygous for the same haplotype as scaleless, indicating that the FGF20 c.535A > T mutation does not lie within a rare haplotype.