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Effect of BPA on morphology. After antral follicles were exposed to DMSO (A, C), BPA at 100 μg/ml (B,D), estradiol at 10 nM (E), or BPA at 100 μg/ml plus estradiol at 10 nM (F) for 48 and/or 120 h, the follicles were removed from culture, and atresia was observed as described in Materials and Methods. Red arrows indicate apoptotic bodies. *P ≤ 0.05 from DMSO control. Original magnification ×40.
