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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Biochemistry. 2012 Sep 21;51(39):7676–7684. doi: 10.1021/bi3009888

Figure 2.

Figure 2

Membrane disruption induced by IAPP. (A) Release of 6-carboxyfluorescein from large unilamellar vesicles (LUVs) composed of 250 µM POPC/POPS (7/3 molar ratio), POPE/POPC/POPS (3/4/3), or POPC/POPS/LysoPC (6.8/3/0.2) induced by 2.5 µM IAPP. The initial phase of membrane disruption was fitted with a double exponential curve (dotted lines). The presence of POPE decreases the amount of dye released during the first phase and markedly increases the efficiency of dye release during the second phase. (B) Release of 6-carboxyfluorescein from seeded fibril growth. Error bars indicate the standard error of measurement. All experiments were performed in triplicate at 25 °C in 10 mM phosphate buffer, 100 mM NaCl, pH 7.4.