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. Author manuscript; available in PMC: 2013 Oct 2.
Published in final edited form as: Biochemistry. 2012 Sep 21;51(39):7676–7684. doi: 10.1021/bi3009888

Figure 4.

Figure 4

Binding of prefibrillar IAPP to lipid vesicles. Changes in molar ellipicity at 222 nm arising from the coil-to-helix conformational change upon membrane binding as a function of the lipid concentration. 25 µM of freshly dissolved IAPP was titrated with the indicated concentrations of 7/3 POPC/POPS (filled circles) and 3/4/3 POPE/POPC/POPS (open circles) LUVs. The final conformation of IAPP is similar in both membranes (Fig. S1). Lines represent a binding isotherm to each dataset. Experiments were performed at 25 °C in 10 mM phosphate buffer, 100 mM NaF pH 7.4. Measurements represent the average of 30 measurements over 30 seconds, error bars indicate the standard deviation of this measurement.