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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Ann Neurol. 2012 Sep;72(3):395–405. doi: 10.1002/ana.23606

Figure 1.

Figure 1

Diagrammatic representation of the lentiviral construct engineered to induce oligodendrocyte apoptosis. (A) Schematic of the viral construct in which an oligodendrocyte-specific promoter sequence (pMBP) drives expression of a suicide gene that is a chemically-inducible analogue of the endogenous Caspase 9 (iCP9). The reporter GFP was expressed downstream driven from a constitutive viral promoter. (B) During canonical apoptosis Caspase 9 dimerizes through binding to APAF-1 to initiate the apoptotic cascade while in the inducible model the N-terminal domain of Caspase 9 (blue) was modified with removal of the APAF-1 binding site and replacement with a CID binding domain to allow chemically-induced dimerization (CID, green). LTR = long terminal repeat; pMBP = promoter for myelin basic protein; pMND = promoter from modified murine leukemia virus; GFP = green fluorescent protein; APAF = apoptotic protease activating factor.