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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Ann Neurol. 2012 Sep;72(3):395–405. doi: 10.1002/ana.23606

Figure 4.

Figure 4

Injection of CID induced the apoptosis of iCP9 expressing oligodendrocytes in vivo. One week after lentiviral injection into the corpus callosum of adult rats, CID was injected distally into the ipsilateral ventricle to activate iCP9-triggered apoptosis. (A) One day post-CID, there was a significant decrease in the number of GFP+/CC1+ oligodendrocytes compared with either the contralateral hemisphere or to vehicletreated controls. (B) Quantitation and comparison of the reduction of CC1+ cells and relative proportion of GFP+ cells expressing CC1. The total number of CC1+ cells was reduced by approximately 50% after CID exposure. Likewise the proportion of GFP+ cells that express CC1 was reduced by approximately 50% after CID treatment. (C) Double labeling with cleaved caspase 3 and CC1 as well as TUNEL labeling confirmed the induction of apoptosis following CID exposure. Scale bar = 25 µm. p < 0.05.