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. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: Dev Dyn. 2011 May;240(5):1212–1222. doi: 10.1002/dvdy.22634

Figure 3. Expression of the reporter gene in ozd mutant limbs indicates the presence of a cohort of ZPA precursor cells in the early limb.

Figure 3

Limbs at stage 23–24 were harvested from equivalently staged normal (A, B, C) or ozd (D, E, F) embryos electroporated with the ZRS-cShh-lacZ construct. The same limb is shown from ventral (A, D), dorsal (B, E), or posterior (C, F) views to visualize the extent of β-gal activity. In posterior views, ventral is to the top and dorsal is to the bottom. Shh mRNA staining in normal limb buds is also shown (G, H) for reference. Note the expanded β-gal staining domain in the ozd limb buds (indicated by white and black arrowheads). Scale bars are included for comparison between samples. St refers to Hamburger and Hamilton 1951, stages of chick development.