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Optical spectra obtained in single-turnover experiment. The reaction mixture contained 30 mM potassium phosphate buffer, pH 6.9, 25 μM 6,7-dimethyl-8-ribityllumazine, and 4 μM riboflavin synthase. (A) Wild type. (B) S41A mutant. Spectra were obtained at 0, 0.025, 0.08, 0.17, 0.3, and 5 min (A) or at 0, 0.08, 3, 6, 15, 30, and 240 min (B). Arrows indicate the optical transient at 312 nm.
