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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer. 2011 Apr 14;117(21):4925–4938. doi: 10.1002/cncr.26116

Figure 4. Correlations of VEGFA gene amplification, VEGFA protein, microvascular density (MVD), and disease-free survival in human osteosarcoma.

Figure 4

A: Strong positive expression of VEGFA protein was detected by immunohistochemical staining, and MVD was measured by scoring CD34-positive cells. Magnification, ×40. Upper panel: VEGFA protein; lower panel: CD34. B: Numbers of MVD in the VEGFA protein–positive cases were significantly higher than in the negative ones. C: Kaplan-Meier survival analysis showed that positive VEGF expression was significantly associated with adverse tumor-free survival. D: Concordance of VEGFA gene amplifications with VEGFA protein expression. E: Kaplan-Meier survival analysis showed that VEGFA gene amplifications were significantly associated with adverse tumor-free survival. F: Kaplan-Meier survival analysis showed that high VEGFA was significantly associated with adverse tumor-free survival.