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Experimental protocol diagram 2. Forty-eight male SD rats were randomly divided into 2 groups (n = 24 each): CON, EA group. At 24 h after the last EA pretreatment and 2 h, 24 h, 72 h after ischemia/reperfusion (red arrow), the rats (n = 6 for each time point) from 2 groups were anesthetized and killed. RT-PCR and Western blot analysis were performed.
