Table 1.

Freezing protocols used

Protocol	Description	Duration [min]	References
1	0,3°C/min to −100°C	347
	Store in vapour phase at −134°C		[47]*
2	1°C/min to −80°C	84	[46-50]
	Store in vapour phase at −134°C
3	1°C/min to - 30°C	44
	5°C/min to −80°C		[44,51]*
	xStore in vapour phase at −134°C
4	1°C/min to −20°C	34
	5°C/min to −40°C
	10°C/min to −80°C		Designed
	20°C/min to −100°C
	Store in vapour phase at −134°C
5	1°C/min to −6°C	26
	25°C/min to −50°C		Designed
	10°C/min to −90°C
	Store in vapour phase at −134°C
6	Directly into the vapour phase	0
7	99°C/min to −100°C	2
	Store in vapour phase at −134°C		[35,52]*

* Modified.