Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Jun;79(12):3853–3857. doi: 10.1073/pnas.79.12.3853

Herpesvirus-specific RNA and protein in carcinoma of the uterine cervix.

J K McDougall, C P Crum, C M Fenoglio, L C Goldstein, D A Galloway
PMCID: PMC346526  PMID: 6285365

Abstract

Cloned probes of herpes simplex virus type 2 DNA were used in cytological hybridization experiments to detect herpesvirus RNA transcripts in the neoplastic cells of tumors of the uterine cervix. Virus-specific RNA was shown to represent transcription of limited regions of the genome, of which one is known to code for a DNA-binding protein that can be found by immunoperoxidase staining in the neoplastic cells of these tumors and has also been detected in cells transformed in vitro by this virus.

Full text

PDF
3853

Images in this article

3854Image
on p.3854
3854Image
on p.3854
3855Image
on p.3855
3855Image
on p.3855
3855Image
on p.3855
3855Image
on p.3855
3855Image
on p.3855
3855Image
on p.3855
3856Image
on p.3856

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adam E., Kaufman R. H., Melnick J. L., Levy A. H., Rawls W. E. Seroepidemiologic studies of herpesvirus type 2 and carcinoma of the cervix. IV. Dysplasia and carcinoma in situ. Am J Epidemiol. 1973 Aug;98(2):77–87. doi: 10.1093/oxfordjournals.aje.a121541. [DOI] [PubMed] [Google Scholar]
  2. Anzai T., Dreesman G. R., Courtney R. J., Adam E., Rawls W. E., Benyesh-Melnick M. Antibody to herpes simplex virus type 2-induced nonstructural proteins in women with cervical cancer and in control groups. J Natl Cancer Inst. 1975 May;54(5):1051–1059. [PubMed] [Google Scholar]
  3. Camacho A., Spear G. Transformation of hamster embryo fibroblasts by a specific fragment of the herpes simplex virus genome. Cell. 1978 Nov;15(3):993–1002. doi: 10.1016/0092-8674(78)90283-0. [DOI] [PubMed] [Google Scholar]
  4. Conley A. J., Knipe D. M., Jones P. C., Roizman B. Molecular genetics of herpes simplex virus. VII. Characterization of a temperature-sensitive mutant produced by in vitro mutagenesis and defective in DNA synthesis and accumulation of gamma polypeptides. J Virol. 1981 Jan;37(1):191–206. doi: 10.1128/jvi.37.1.191-206.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Copple C. D., McDougall J. K. Clonal derivatives of a herpes type 2 transformed hamster cell line (333-8-9): cytogenetic analysis, tumorigenicity and virus sequence detection. Int J Cancer. 1976 Apr 15;17(4):501–510. doi: 10.1002/ijc.2910170413. [DOI] [PubMed] [Google Scholar]
  6. Docherty J. J., Subak-Sharpe J. H., Preston C. M. Identification of a virus-specific polypeptide associated with a transforming fragment (BglII-N) of herpes simplex virus type 2 DNA. J Virol. 1981 Oct;40(1):126–132. doi: 10.1128/jvi.40.1.126-132.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dreesman G. R., Burek J., Adam E., Kaufman R. H., Melnick J. L., Powell K. L., Purifoy D. J. Expression of herpesvirus-induced antigens in human cervical cancer. Nature. 1980 Feb 7;283(5747):591–593. doi: 10.1038/283591a0. [DOI] [PubMed] [Google Scholar]
  8. Flannery V. L., Courtney R. J., Schaffer P. A. Expression of an early, nonstructural antigen of herpes simplex virus in cell transformed in vitro by herpes simplex virus. J Virol. 1977 Jan;21(1):284–291. doi: 10.1128/jvi.21.1.284-291.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Frenkel N., Locker H., Cox B., Roizman B., Rapp F. Herpes simplex virus DNA in transformed cells: sequence complexity in five hamster cell lines and one derived hamster tumor. J Virol. 1976 Jun;18(3):885–893. doi: 10.1128/jvi.18.3.885-893.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Frenkel N., Roizman B., Cassai E., Nahmias A. A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784–3789. doi: 10.1073/pnas.69.12.3784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Galloway D. A., Copple C. D., McDougall J. K. Analysis of viral DNA sequences in hamster cells transformed by herpes simplex virus type 2. Proc Natl Acad Sci U S A. 1980 Feb;77(2):880–884. doi: 10.1073/pnas.77.2.880. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Galloway D. A., McDougall J. K. Transformation of rodent cells by a cloned DNA fragment of herpes simplex virus type 2. J Virol. 1981 May;38(2):749–760. doi: 10.1128/jvi.38.2.749-760.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Galloway D. A., Swain M. Cloning of Herpes simplex virus 2 DNA fragments in a plasmid vector. Gene. 1980 Nov;11(3-4):253–257. doi: 10.1016/0378-1119(80)90065-7. [DOI] [PubMed] [Google Scholar]
  14. Gilman S. C., Docherty J. J., Clarke A., Rawls W. E. Reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls. Cancer Res. 1980 Dec;40(12):4640–4647. [PubMed] [Google Scholar]
  15. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hollinshead A. C., Tarro G. Soluble membrane antigens of lip and cervical carcinomas: reactivity with antibody for herpesvirus nonvirion antigens. Science. 1973 Feb 16;179(4074):698–700. doi: 10.1126/science.179.4074.698. [DOI] [PubMed] [Google Scholar]
  17. Jariwalla R. J., Aurelian L., Ts'o P. O. Tumorigenic transformation induced by a specific fragment of DNA from herpes simplex virus type 2. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2279–2283. doi: 10.1073/pnas.77.4.2279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kaufman R. H., Dreesman G. R., Burek J., Korhonen M. O., Matson D. O., Melnick J. L., Powell K. L., Purifoy D. J., Courtney R. J., Adam E. Herpesvirus-induced antigens in squamous-cell carcinoma in situ of the vulva. N Engl J Med. 1981 Aug 27;305(9):483–488. doi: 10.1056/NEJM198108273050903. [DOI] [PubMed] [Google Scholar]
  19. Kawana T., Yoshino K., Kasamatsu T. Estimation of specific antibody to type 2 Herpes simplex virus among patients with carcinoma of the uterine cervix. Gan. 1974 Oct;65(5):439–445. [PubMed] [Google Scholar]
  20. Kessler I. I. Venereal factors in human cervical cancer: evidence from marital clusters. Cancer. 1977 Apr;39(4 Suppl):1912–1919. doi: 10.1002/1097-0142(197704)39:4+<1912::aid-cncr2820390829>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  21. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  22. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  23. Lewis J. G., Kucera L. S., Eberle R., Courtney R. J. Detection of herpes simplex virus type 2 glycoproteins expressed in virus-transformed rat cells. J Virol. 1982 Apr;42(1):275–282. doi: 10.1128/jvi.42.1.275-282.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Maitland N. J., Kinross J. H., Busuttil A., Ludgate S. M., Smart G. E., Jones K. W. The detection of DNA tumour virus-specific RNA sequences in abnormal human cervical biopsies by in situ hybridization. J Gen Virol. 1981 Jul;55(Pt 1):123–137. doi: 10.1099/0022-1317-55-1-123. [DOI] [PubMed] [Google Scholar]
  25. Maniatis T., Kee S. G., Efstratiadis A., Kafatos F. C. Amplification and characterization of a beta-globin gene synthesized in vitro. Cell. 1976 Jun;8(2):163–182. doi: 10.1016/0092-8674(76)90001-5. [DOI] [PubMed] [Google Scholar]
  26. McDougall J. K., Galloway D. A., Fenoglio C. M. Cervical carcinoma: detection of herpes simplex virus RNA in cells undergoing neoplastic change. Int J Cancer. 1980 Jan 15;25(1):1–8. doi: 10.1002/ijc.2910250102. [DOI] [PubMed] [Google Scholar]
  27. Melnick J. L., Adam E., Lewis R., Kaufman R. H. Cervical cancer cell lines containing herpesvirus markers. Intervirology. 1979;12(2):111–114. doi: 10.1159/000149076. [DOI] [PubMed] [Google Scholar]
  28. Moar M. H., Jones K. W. Detection of virus-specific DNA and RNA base-sequences in individual cells transformed or infected by adenovirus type 2. Int J Cancer. 1975 Dec 15;16(6):998–1007. doi: 10.1002/ijc.2910160613. [DOI] [PubMed] [Google Scholar]
  29. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Guest B. A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. II. Women with cervical cancer. Am J Epidemiol. 1970 Jun;91(6):547–552. doi: 10.1093/oxfordjournals.aje.a121166. [DOI] [PubMed] [Google Scholar]
  30. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979 Feb;93(1):111–126. doi: 10.1016/0042-6822(79)90280-0. [DOI] [PubMed] [Google Scholar]
  31. Pacsa A. S., Kummerländer L., Pejtsik B., Pali K. Herpesvirus antibodies and antigens in patients with cervical anaplasia and in controls. J Natl Cancer Inst. 1975 Oct;55(4):775–781. doi: 10.1093/jnci/55.4.775. [DOI] [PubMed] [Google Scholar]
  32. Pagano J. S. Diseases and mechanisms of persistent DNA virus infection: latency and cellular transformation. J Infect Dis. 1975 Aug;132(2):209–223. doi: 10.1093/infdis/132.2.209. [DOI] [PubMed] [Google Scholar]
  33. Powell K. L., Littler E., Purifoy D. J. Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein. J Virol. 1981 Sep;39(3):894–902. doi: 10.1128/jvi.39.3.894-902.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Powell K. L., Littler E., Purifoy D. J. Nonstructural proteins of herpes simplex virus. II. Major virus-specific DNa-binding protein. J Virol. 1981 Sep;39(3):894–902. doi: 10.1128/jvi.39.3.894-902.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Rapp F., Westmoreland D. Cell transformation by DNA-containing viruses. Biochim Biophys Acta. 1976 Jul 16;458(2):167–211. doi: 10.1016/0304-419x(76)90005-6. [DOI] [PubMed] [Google Scholar]
  36. Rawls W. E., Tompkins W. A., Figueroa M. E., Melnick J. L. Herpesvirus type 2: association with carcinoma of the cervix. Science. 1968 Sep 20;161(3847):1255–1256. doi: 10.1126/science.161.3847.1255. [DOI] [PubMed] [Google Scholar]
  37. Rawls W. E., Tompkins W. A., Melnick J. L. The association of herpesvirus type 2 and carcinoma of the uterine cervix. Am J Epidemiol. 1969 May;89(5):547–554. doi: 10.1093/oxfordjournals.aje.a120967. [DOI] [PubMed] [Google Scholar]
  38. Reed C. L., Cohen G. H., Rapp F. Detection of a virus-specific antigen on the surface of herpes simplex virus-transformed cells. J Virol. 1975 Mar;15(3):668–670. doi: 10.1128/jvi.15.3.668-670.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Reyes G. R., LaFemina R., Hayward S. D., Hayward G. S. Morphological transformation by DNA fragments of human herpesviruses: evidence for two distinct transforming regions in herpes simplex virus types 1 and 2 and lack of correlation with biochemical transfer of the thymidine kinase gene. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):629–641. doi: 10.1101/sqb.1980.044.01.066. [DOI] [PubMed] [Google Scholar]
  40. Richart R. M. Cervical intraepithelial neoplasia. Pathol Annu. 1973;8:301–328. [PubMed] [Google Scholar]
  41. Roizman B. The structure and isomerization of herpes simplex virus genomes. Cell. 1979 Mar;16(3):481–494. doi: 10.1016/0092-8674(79)90023-0. [DOI] [PubMed] [Google Scholar]
  42. Smith C. C., Gupta P. K., Aurelian L. Expression of herpesvirus-induced antigens in human cervical cancer. Nature. 1981 Jul 23;292(5821):388–389. doi: 10.1038/292388b0. [DOI] [PubMed] [Google Scholar]
  43. Sprecher-Goldberger S., Thiry L., Gould I., Fassin Y., Gompel C. Increasing antibody titers to herpes simplex virus type 2 during follow-up of women with cervical dysplasia. Am J Epidemiol. 1973 Feb;97(2):103–110. doi: 10.1093/oxfordjournals.aje.a121486. [DOI] [PubMed] [Google Scholar]
  44. Thomas D. B., Rawls W. E. Relationship of herpes simplex virus type-2 antibodies and squamous dysplasia to cervical carcinoma in situ. Cancer. 1978 Dec;42(6):2716–2725. doi: 10.1002/1097-0142(197812)42:6<2716::aid-cncr2820420629>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  45. Walboomers J. M., Schegget J. T. A new method for the isolation of herpes simplex virus type 2 DNA. Virology. 1976 Oct 1;74(1):256–258. doi: 10.1016/0042-6822(76)90151-3. [DOI] [PubMed] [Google Scholar]
  46. Zweig M., Heilman C. J., Jr, Rabin H., Hampar B. Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. J Virol. 1980 Sep;35(3):644–652. doi: 10.1128/jvi.35.3.644-652.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. zur Hausen H., Schulte-Holthausen H., Wolf H., Dörries K., Egger H. Attempts to detect virus-specific DNA in human tumors. II. Nucleic acid hybridizations with complementary RNA of human herpes group viruses. Int J Cancer. 1974 May 15;13(5):657–664. doi: 10.1002/ijc.2910130510. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES