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. 2012 Oct 5;7(10):e46890. doi: 10.1371/journal.pone.0046890

Figure 4. Specificity and cross-reaction analysis of the miRNA assay.

Figure 4

(A&B) Relative level of PCR products using a mismatched primer compared to the perfectly matched primer in the normal program (annealing temperature 55°C) and in the high-stringency program (annealing temperature 62°C) for amplifying the target hsa-miR-32. Each column represents the mean (± SD) of three measurements. (C&D) Cross-reaction of the human let-7 family assays (annealing temperature 56°C). The percentage of cross-reaction values was calculated based on the C q difference between assay-specific and nonspecific miRNA targets. The PCR annealing temperature was raised to 60°C to distinguish let-7a and let-7c.