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. 2012 Oct 5;7(10):e46682. doi: 10.1371/journal.pone.0046682

Figure 1. Dose-response and time-course experiments.

Figure 1

a. Dose-response experiment to r-hLH and r-hCG in COS7/LHCGR in the presence of 500 µM IBMX. Total cAMP was measured after 3 hours of incubation and the results of four independent experiments were plotted (Mean±SEM). b. Time-course experiment performed by continuous incubation of COS7/LHCGR for different time-points in the presence of 500 µM IBMX and gonadotropins at ED50 doses (500 pM r-hLH; 100 pM r-hCG). Intracellular cAMP was measured (n = 3; Mean±SEM). Asterisk indicates the significant differences of hLH vs hCG (t-test; p<0.05).