Figure 3. Long-term kinetics of intracellular cAMP production in hGLC continuously exposed to r-hLH or r-hCG.
hGLC were cultured in the presence IBMX (500 µM) under continuous stimulation by r-hLH (500 pM) or r-hCG (100 pM) for up to 36 hours: (a) 0–12 hours, (b) 13–24 hours, (c) 25–36 hours. Results are means±SEM (n = 3). Asterisks indicate significant differences between hCG and hLH at the given time point (n = 3; t-test; p<0.05).