Table 1. Primer sequences used in real-time PCR experiments.
Gene | Oligonucleotide sequences | Product length (bp) | NCBI Ref. Sequence | Protein name |
AREG | F:GACACCTACTCTGGGAAGCG | 121 | NM_001657.2 | Amphiregulin |
R:AAGGCATTTCACTCACAGGG | ||||
EREG | F:TACTGCAGGTGTGAAGTGGG | 139 | NM_001432.2 | Epiregulin |
R:TGGAACCGACGACTGTGATA | ||||
NRG1* | F:CCCCGATTGAAAGAGATGAA | 116 | NM_001159999.1 | Neuregulin 1 |
R:TTCCCATTCTTGAACCACTTG | ||||
CYP19A1 | F:CCCTTCTGCGTCGTGTCAT | 86 | NM_000103.3 | Aromatase |
R:GATTTTAACCACGATAGCACTTTCG | ||||
RPS7** | F: AATCTTTGTTCCCGTTCCTCA | 135 | NM_001011.3 | Ribosomal protein S7 |
R: CGAGTTGGCTTAGGCAGAA |
Primer chosen to include all NRG1 transcript variants, verified by NCBI BLAST.
Endogenous control gene.