Table 4. qRT-PCR validation of selected up-regulated genes in HAmCqG8 as identified by the RNASeq quantification.
| The ratio of gene overexpression in HAmCqG8 † | ||||
| Gene category | Vectorbase # | Annotation§ | RNASeq | qPCR |
| Cytochrome P450 | CPIJ002538 | CYP6AG12‡ | 3.7 | 2.1†† |
| CPIJ005959 | CYP6AA7‡ | 7.3 | 2.1 | |
| CPIJ005957 | CYP6AA9‡ | 6.6 | 2.8 | |
| CPIJ010546 | CYP9J34‡ | 13.4 | 2.9 | |
| CPIJ009478 | CYP4D42v1‡ | 2.4 | 3.2 | |
| CPIJ005956 | CYP6BZ2‡ | 3.3 | 3.7 | |
| CPIJ010537 | CYP9J45‡ | 4.8 | 3.8 | |
| CPIJ012470 | CYP9AL1‡ | 9.2 | 3.8 | |
| CPIJ014218 | CYP9M10‡ | 3.7 | 4.2 | |
| CPIJ010225 | CPY12F7‡ | 3.9 | 5.2 | |
| CPIJ010227 | CYP12F13‡ | 7.1 | 5.2 | |
| CPIJ010543 | CYP9J40‡ | 7.2 | 6.0 | |
| CPIJ005955 | CYP6P14‡ | 8.2 | 6.3 | |
| CPIJ020229 | CYP4D42v2‡ | 2.4 | 7.0 | |
| Protease | CPIJ002139 | HzC4 chymotrypsinogen | 4.3 | 1.1±0.11 |
| CPIJ002130 | kallikrein-7 | 2.4 | 1.5±0.50 | |
| CPIJ013319 | metalloproteinase | 3.5 | 1.5±0.10 | |
| CPIJ009106 | angiotensin-converting enzyme | 2.7 | 1.5±0.84 | |
| CPIJ001240 | cathepsin B-like thiol protease | 5.3 | 1.6±0.46 | |
| CPIJ019428 | trypsin 2 | 3.4 | 1.6±0.04 | |
| CPIJ004086 | angiotensin-converting enzyme | 5.7 | 1.7±0.62 | |
| CPIJ008873 | prolylcarboxypeptidase | 3.5 | 1.7±1.09 | |
| CPIJ002135 | trypsin alpha-4 | 5.9 | 1.8±0.84 | |
| CPIJ016012 | tryptase-2 | 2.2 | 1.8±0.10 | |
| CPIJ002142 | chymotrypsin BI | 2.8 | 2.0±0.42 | |
| CPIJ006803 | zinc metalloproteinase nas-7 | 4.5 | 2.0±0.21 | |
| CPIJ007383 | endothelin-converting enzyme 1 | 2.5 | 2.1±1.08 | |
| CPIJ010224 | metalloproteinase | 2.9 | 2.4±0.74 | |
| CPIJ014523 | elastase-3A | 3.0 | 2.4±0.71 | |
| CPIJ019029 | metalloproteinase | 2.6 | 3.6±0.70 | |
| CPIJ002128 | mast cell protease 2 | 16.1 | 3.6±0.04 | |
| CPIJ006542 | chymotrypsin-2 | 19.7 | 5.4±1.84 | |
| CPIJ010805 | carboxypeptidase A1 | 4.4 | 6.9±3.72 | |
| CPIJ006076 | hypodermin-B | 17.0 | 11.6±4.96 | |
| CPIJ001743 | carboxypeptidase A2 | 5.4 | 16.3±5.48 | |
| CPIJ003623 | coagulation factor XII | 7.1 | 54.2±19.79 | |
| CPIJ001742 | zinc carboxypeptidase | 3.0 | 99.5±19.35 | |
| CPIJ009594 | nephrosin | 21.7 | 144.3±13.8 | |
§
Culex quinquefasciatus genome, Johannesburg strain CpipJ1.2, June 2008; http://cquinquefasciatus.vectorbase.org/.
†
The ratio of gene overexpression in HAmCqG8 compared to HAmCqG0.
‡
Annotations for cytochrome P450 genes were taken from the most current annotation based on: Nelson (2009) The Cytochrome P450 Homepage. Human Genomics 4, 59-65: http://drnelson.uthsc.edu/CytochromeP450.html.
††
Data reprinted from Yang and Liu, 2011.