Figure 4. SIRT1 knockdown reduces the ability of ω-3 PUFAs to deacetylate NF-κB in macrophages.

A representative blot was shown in (A), and the blots were quantitated with a Li-COR Odyssey Infrared System (B). The SIRT1 knockdown or control macrophages were transfected with expression vectors for p65 or p300, and were then treated with DHA (100 µM) for 24 hours. Acetylation of p65 at lysine310 and SIRT1 protein were measured by immunoblotting with specific antibody. Groups labeled with the same superscripts are not statistically different from each other. Groups labeled with different superscripts are statistically different from each other.