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. 2012 Oct 5;7(10):e46090. doi: 10.1371/journal.pone.0046090

Figure 2. The targeting sites of TRIB2–3′UTR and structural map of the vector.

Figure 2

(A) The targeting sites on TRIB2–3′UTR. miR-511, miR-1297 and miR-26a are shown. Each miRNA is aligned with the mRNA of human TRIB2 with the nucleotide position on the TRIB2 mRNA indicated. Vertical lines indicate identity; gaps indicate mismatch. (B) Structural map of the pcDNA-GFP-TRIB2–3′UTR vector. 3′-UTR of TRIB2 is indicated downstream of the GFP gene. Pcmv: cmv promoter. GFP: green fluorescent protein. TRIB2–3′UTR, 3′-UTR of the TRIB2 gene. (C) Real-time PCR detection. 24 h after lung adenocarcinoma cells were treated with miRNAs, higher miR-511 and miR-1297 levels were found in the transfected cells than in the non-transfected cells.