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. 2001 Jun 19;98(13):7241–7246. doi: 10.1073/pnas.131132198

Figure 1.

Figure 1

(A) Immunoblotting analyses of microsome preparations expressing the indicated constructs. Pep3 recognizes TAP1 and T1MT2C. 148.3 and anti-his recognize TAP1 and T2MT1C. 435.3 recognizes TAP2 and T2MT1C. Anti-TAP2 recognizes TAP2 and T1MT2C. (B) Complex formation between T1MT2C and T2MT1C. Microsomes expressing the indicated constructs were directly analyzed by immunoblotting assays with 148.3 and anti-TAP2 antiserum (Top and Middle) or were first detergent solubilized, immunoprecipitated with 148.3, and analyzed by immunoblotting with anti-TAP2 (Bottom). (C) Complex formation between TAP1 and T1MT2C and between TAP2 and T2MT1C. Microsomes expressing the indicated constructs were directly analyzed by immunoblotting assays with anti-his or anti-TAP2 (Top and Middle) or were first detergent solubilized, immunoprecipitated with anti-his, followed by immunoblotting analyses with anti-TAP2 (Bottom).