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. 2001 Jun 19;98(13):7241–7246. doi: 10.1073/pnas.131132198

Figure 2.

Figure 2

Nucleotide binding by chimeric and wild-type TAP constructs. (A) Cells expressing the indicated TAP constructs were lysed, immunoprecipitated with anti-his (lanes 1 and 5), or incubated with ATP (lanes 2 and 6), ADP (lanes 3 and 7) or AMP-agarose (lanes 4 and 8) beads, followed by immunoblotting analyses with anti-his. (B–F) Same procedure as in A was followed except that the microsome preparations rather than cell lysates were used to compare expression levels. Immunoblotting analysis was carried out with (B) anti-TAP2, (C and E) Pep3, and (D and F) 435.3.