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Peptide translocation assays. Microsomal vesicles expressing the indicated TAP complexes were assayed for their ability to import 125I-labeled RRYNASTEL. Bar graphs represent the average of experiments done in triplicate. Translocation in the presence of ATP is indicated by solid bars and in the presence of Apyrase by open bars. (Insets) Immunoblots of various constructs used in translocation experiments, presented in the same sequence as the translocation experiments.
