Table 1. Overexpression of TFPI in breast cancer cells.
TFPI mRNA FC of control | Total TFPI ag FC of control | ||
SK-BR-3 | pTOPO-TFPIα | 34±2.1 | 6.4±0.1 |
pTOPO-TFPIβ | 55±6.4 | 17.3±0.1* | |
pTOPO (control) | 1±0.1 | 1.0±0.1 |
Overexpression of TFPIα or TFPIβ in SK-BR-3 breast cancer cells, displayed as a summary of previously reported data [18]. mRNA levels were quantified using qRT-PCR. Protein levels were measured in cell medium using total TFPI ELISA. Results are presented relatively as mean fold change (FC) of control (n = 3± SD). * Relative increase in TFPI antigen measured in cell medium after PI-PLC treatment.