Figure 4. Addition of IL-4 reduces IL-12p40 and IL-10 production by BMMφ whereas neutralization of IL-4 does not alter cytokine production.

(a–c) IFN-γ-primed BMMφ (10⁵/well) from WT (a–c) and IL-4Rα−/− (c) mice were stimulated with LPS (10 ng/ml) in the presence or absence of opsonized SRBC (IC), IL-4 (3 ng/ml), or neutralizing anti-IL-4 mAb (1 µg/ml) for 24 hours. In (c) only cultures with exogenous IL-4 (3 ng/ml) are shown. Cytokine production was measured in culture supernatants by ELISA. Shown are the means and SEM of triplicate wells from 1 of 3 representative experiments. *p<0.001 LPS or LPS + anti-IL-4 versus all other groups, **p<0.001 LPS + IL-4 versus all other groups, and ***p<0.01 LPS versus LPS + IL-4 or LPS + IC by one-way ANOVA with Bonferroni’s post-test. For (c) *p<0.05 compared to medium by Dunnett’s Multiple Comparison Test. (d) IFN-γ-primed BMMφ (10⁵/well) from heterozygous G4 mice were stimulated as above (a–c) and IL-4 production assessed by flow cytometry. % medium  =  (geoMFI of sample/geoMFI of medium)×100. Shown are the means and SEM of triplicate wells from 3 experiments. *p<0.01 compared to medium by Dunnett’s Multiple Comparison Test.