Figure 1. Expression of NR4A1 dominant negative mutants inhibits IGF1R-induced death.

HEK293 cells were co-transfected with the indicated plasmids. Co-expression of dominant negative mutants NR4A1ΔDBD or NR4A1ΔN152 significantly reduced cell death. A, Percentage of cell death was determined by trypan blue exclusion 24 hr after transfection. Bars indicate standard error. ***, p<0.001, n = 4. B, Morphology of the rescued cells observed by phase contrast microscopy.