Table 5. Sequence of Primers Used for RT-PCR Detection of Gene Expression.
| Gene | Forward primer(5→3) | Reverse primer(5→3) | Amplicon size (bp) | Annealing temperature (°C) |
| RALDH1 | ACTGTGTCATCTGCTCTG | TTACTCTGCTGGCTTCTT | 200 | 60 |
| RALDH2 | ACATCAACAAGGCTCTCA | CCAAACTCACCCATTTCTC | 139 | 60 |
| RALDH3 | AGAGGGCTGTTCATCAAA | TGCTGTGAGTCCATAGTC | 159 | 60 |
| RARα | AACAACAGCTCAGAACAAC | CGAACTCCACAGTCTTAATG | 97 | 60 |
| RARγ | AGACTTTTCCCTCACTCTG | TTCGCAAACTCCACAATC | 235 | 60 |
| RARβ | CTTGGGCCTCTGGGACAAAT | TGGCGAACTCCACGATCTTAAT | 68 | 60 |
| GAPDH | AACCTGCCAAGTATGATGA | GGAGTTGCTGTTGAAGTC | 119 | 60 |