Table 1. Primer sets used for PCR and RT-PCR to detect hHO-1 in transgenic piglets.
| Gene | Primer sequence (5′–3′) | Annealing temperature | PCR fragment size |
| HO-11 | F:ATGGAGCGTCCGCAACCCGACAG | 62°C | 867 bp |
| R:TCACATGGCATAAAGCCCTACAG | |||
| GAPDH2 | F:CTA CTG CCA ACG TGT CGG TT | 60°C | 128 bp |
| R:CTA CTG CCA ACG TGT CGG TT | |||
| iNOS3 | F:AGA GCC TCT GGA CCT CAA CA | 60°C | 136 bp |
| R:CTC ACA GCA GAG TTC CAC CA | |||
| RANTES4 | F:CAT GGC AGC AGT CGT CTT TA | 60°C | 165 bp |
| R:GGG ACA AGA GCA AGA AGC AG | |||
| IP-105 | F:CAT GGA TTG CAG TCA CCA AG | 60°C | 73 bp |
| R:AAC AGC TCG GGA TGA TGA AC |
1
Homo sapiens heme oxygenase 1.
2
Glyceraldehyde-3-phosphate dehydrogenase.
3
Inducible nitric oxide synthase.
4
Regulated upon Activation, Normal T-cell Expressed, and Secreted (CCL5).
5
Interferon gamma-induced protein 10 (CXCL10).