Table I.
Transition temperatures of synthetic PEs
Phospholipid | Tm | Th | Recognition by 24.8.Ac |
---|---|---|---|
°Ca | °Cb | ||
Di-12:0 PE | 17 | 44 | Yes |
Di-14:0 PE | 50 | 123d | No |
Di-16:0 PE | 63 | 118 | No |
Di-18:0 PE | 74 | 100 | No |
Di-16:1[cis] PE | −33 | 0 | Yes |
Di-18:1[cis] PE | −16 | 10 | Yes |
Di-18:1[trans] PE | 38 | 64 | No |
Di-18:2[cis] PE | −40 | −15 | Yes |
Di-18:3[cis] PE | NAe | −30 | Yes |
16:0–18:1[cis] PE | 25 | 71 | Yes |
Tm is the transition temperature from the gel to fluid liquid crystalline phase.
Th is the transition temperature from the lamellar to hexagonal (HII) phase.
Recognition by the 24.8.A iNKT cell hybridoma in the plate-bound CD1d assay (based on data from Fig. 5).
All transition temperatures are approximate and for lipid in aqueous solution (water or physiological buffer), with the exception of di-14:0 PE, which appears to undergo transition to the hexagonal phase in aqueous solution only at high salt concentrations (e.g., 2.4 M NaCl). Transition temperatures were obtained from LIPIDAT databases (www.lipidat.chemistry.ohio-state.edu).
NA, not available.