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. 2012 Sep 10;109(39):15877–15881. doi: 10.1073/pnas.1209042109

Fig. 2.

Fig. 2.

TARP-TCR–modified T cells secrete IFN-γ in response to peptide-specific stimulation. (A) HLA-A2+ T2 target cells were pulsed for 2 h with the TARP(P5L)4–13 peptide (T2 TARP) or the irrelevant HIV-1pol476–484 peptide (T2 HIV) and cocultured overnight with TARP-TCR–engineered T cells. (B) HLA-A2+ tumor cells (mel526) were transfected with a plasmid encoding either the full-length wild-type TARP protein (mel526 TARP) or the irrelevant PSCA protein (mel526 PSCA) and cocultured overnight with TARP-TCR–engineered T cells. An ELISA was used to measure IFN-γ secretion from TARP-TCR–engineered T cells. Asterisks denote significant difference (***P < 0.001, Student t test). Bars represent mean +SD from three experiments.