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. 2012 Sep 4;109(39):E2587–E2594. doi: 10.1073/pnas.1202789109

Fig. 2.

Fig. 2.

Ultrastructure of the yeast endocytic profiles after interfering with actin and clathrin. (A and D) Immuno-EM micrographs showing representative PM invaginations labeled with immunogolds against Sla1-HA (α-Sla1-HA) or Clathrin (α-Chc1) on (A) WT cells treated with 200 μM LatA or DMSO or (D) chc1Δ cells and the isogenic WT (CHC1). (B and E) Scatter graphs representing the GDPM (gold distance to the plasma membrane) of 120 immunogolds against Sla1-HA vs. the corresponding IL (parameter definition in Fig. 1C) for each of the indicated strains and the experimental conditions described in A and D, respectively. The x axis corresponds to the level of the basal PM. The red dotted line indicates the position of the IT. The gray lines converging at the origin define constant GRP values. Blue and red circles indicate the control strains and the LatA-treated or mutant strains, respectively. (C and F) Histograms showing the relative frequency of Sla1-HA immunogolds from the scatter graphs shown in B and E, respectively, binned according to the length of the corresponding invaginations. Student t test P values comparing the length of Sla1-HA–labeled invaginations or the positional parameters of the corresponding immunogolds are described in SI Appendix, Table S1.