Fig. 3.

Cell dissemination into collagen I is persistent in tumor and transient in normal epithelium. (A–C) Tumor cells disseminate with mesenchymal (black arrowheads) (A), amoeboid (white arrowheads) (B), and collective (black arrow) (C) morphologies. (D and E) Distribution of morphological types of dissemination (D) and fate of disseminated cells in normal and tumor organoids (E) in collagen I. n, total number of disseminated cells observed in each condition. (F and G) Representative frames from DIC time-lapse movies of tumor (F) and normal organoids (G) in collagen I. (H and I) Localization of E-cadherin and DAPI in tumor (H) and normal organoids (I) cultured in Matrigel. (J and K) Localization of E-cadherin and DAPI in tumor (J) and normal organoids (K) cultured in collagen I.