Fig. 5.

Tumor and normal epithelium remain transcriptionally distinct despite morphological similarities induced by the ECM. (A) Schematic description of 3D cultures of normal and tumor murine epithelial fragments in Matrigel and collagen I for mRNA expression analyses. n = at least 3 biological replicates. (B) Complete-linkage hierarchical clustering of the experimental conditions. (C) Principal component analysis of the experimental conditions. (D) Heatmap representation of the 19,693 genes included in the microarray (blue and red indicate lower and higher expression, respectively). (E) Summary of differentially expressed genes with fold changes ≥2 and FDR ≤0.05. (F and G) Genes differentially expressed based on ECM condition in normal (F) and tumor (G). C, collagen I; M, Matrigel.