Skip to main content
. 2012 Sep 10;109(39):15722–15727. doi: 10.1073/pnas.1206077109

Fig. 4.

Fig. 4.

An alternative conformation where the flavin is in the opposite side of the active site, and away from the substrate dUMP binding-site. (A) The flavin binding mode observed in one of the active sites of E144R mutant (PDB code: 4GTD), with the 2Fo-Fc electron density contoured at 1.5σ. (B) The flavin binding mode observed in two of the actives sites of R174K mutant (PDB code: 4GTL), with the 2Fo-Fc electron density contoured at 1.5σ shown for FAD, H53, K174, and H79. Superposition with the wild-type enzyme in complex with dUMP (PDB code: 1O26) is shown in pink.