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TBC1D16 expression alters the appearance of GFP-Rab4A in HeLa cells. HeLa cells were transfected with GFP-Rab4A alone (Left), GFP-Rab4A with WT Myc-TBC1D16 (Middle), or Myc-TBC1D16-R494A (Right). GAP proteins were detected using anti-Myc antibodies (red) after cytosolic depletion using liquid nitrogen. (Scale bar: 10 μm.)
